HINTS & TIPS archive.

Hints & tips from Nucleic Acids Research: FusionAnalyser – a new graphical, event-driven tool for fusion rearrangements discovery.

Hints & tips from American Biotechnologist: Top 8 reasons for high background noise on your western blot.

NextBio uses genomic profiles for biomarker discovery

NextBio has launched NextBio Clinical, extending its existing life sciences platform to translational medicine applications such as biomarker discovery and clinical trial optimization by adding in curated genomic, molecular and clinical profiles from thousands of individual patients to the platform’s existing repository of data from animal and cell line models.

Hints & tips: A fluorescent DNA toolkit. These reagents open up new future applications of Cy3B, including more sensitive single-molecule and cell-imaging studies.

Hints & tips: Characterization of a novel DNA polymerase activity assay. This is a rapid, highly sensitive and quantitative assay capable of measuring DNA polymerase extension activity from purified enzymes or directly from microbial lysates.

Hints & tips: Computer simulations – tools for population and evolutionary genetics. This review of simulation software identifies applications of simulations, evaluates simulator capabilities, provides a guide for their use and summarizes future directions.

Hints & tips: A beginner’s guide to eukaryotic genome annotation. This article provides an overview of the genome annotation process and the available tools and describes some best-practice approaches.

FlexGen has launched its gene searcher tool. The free tool allows researchers and clinicians to search for pre-defined FleXelect kits that target their genes of interest for next generation sequencing. FleXelect in-solution target enrichment kits feature high coverage and superior evenness of enrichment due to double stranded DNA capture technology.

Hints and tips: SHRiMP

Hints and tips: SHRiMP (SHort Read Mapping Package) is a software package for aligning genomic reads against a target genome.

RNAi in action

See RNAi in action with Nature Genetics’ animation and slideshow.